A cross-sectional descriptive study was conducted from 3^rd December 2023 to 2^nd June 2024, at the Obstetrics & Gynecology department of Bilawal Medical College and affiliated Countess of Dufferin Fund Hospital, Jamshoro, Pakistan. Pregnant women from all trimesters, aged 20–40 years, were recruited using non-probability consecutive sampling after obtaining informed consent. Pregnant women having painful legs, peripheral neuropathy, akathisia, nocturnal leg cramps, peripheral vascular disease, pre-gestational diabetes, renal failure, and varicose veins were excluded. RLS was diagnosed based on the 2012 revised International RLS Study Group (IRLSSG) criteria. 5 mL of blood was collected for complete blood count (CBC) and iron studies. Data analysis was performed using SPSS Version 22.